Journal: Veterinary Research
Article Title: Baicalin attenuates PD-1/PD-L1 axis-induced immunosuppression in piglets challenged with Glaesserella parasuis by inhibiting the PI3K/Akt/mTOR and RAS/MEK/ERK signalling pathways
doi: 10.1186/s13567-024-01355-1
Figure Lengend Snippet: The effects of baicalin, levamisole, and BMS-1 on the proportions of CD3 + CD8 + T cells among splenocytes and blood and the proportion of CD3 – CD21 + B cells among splenocytes were measured by flow cytometry. Blood was collected, the spleen was collected, and splenocytes were prepared. The proportions of CD3 + CD8 + T cells and CD3 – CD21 + B cells were determined by flow cytometry. A Proportion of CD3 + CD8 + T cells among splenocytes; B proportion of CD3 + CD8 + T cells among blood cells after 24 h; C proportion of CD3 + CD8 + T cells among blood cells after 48 h; D proportion of CD3 – CD21 + B cells among splenocytes; BA: Baicalin; ## P < 0.01 versus controls; ### P < 0.001 versus controls; *significance at P < 0.05; **significance at P < 0.01; ***significance at P < 0.001.
Article Snippet: Following staining with mouse anti-porcine CD3ε-FITC (Cat. No. 4510-02, Monoclonal), mouse anti-porcine CD4-SPRD (Cat. No. 4515-13, Monoclonal), mouse anti-porcine CD8a-PE (Cat. No. 4520-09, Monoclonal) and mouse anti-porcine CD21-PE (Cat. No. 4530-09, Monoclonal) (all from SouthernBiotech, Birmingham, AL, USA) to gate for CD3 T cells, CD4 T cells, CD8 T cells and CD21 B cells, the cells were stained with CD3ε-FITC-CD4-SPRD, CD3ε-FITC-CD8a-PE or CD3ε-FITC-CD21-PE to generate a four-quadrant gate.
Techniques: Flow Cytometry